Our research strategy is to discover the key cellular processes governed by AMPK and SIK by using tissue-specific knockout mouse models and genetically modified cell lines with the CRISPR-Cas9 technology; our general objective is to identify and validate target proteins and genes by molecularly interrogating established in vivo and in vitro experimental models (through multi-omics analysis), and to propose specific therapeutic approaches by genetic and pharmacological means. Our current efforts are aimed at deciphering the function of AMPK in gastrointestinal tract (Axis 1) and exploring the role of SIK  in the regulation of hepatic glucose production (Axis 2) and assess their utility in diagnosis and efficacy in therapeutics for type 2 diabetes and obesity. The impact of our research will be relevant not only for the purpose of growing scientific knowledge but also for its translation into new therapeutic strategies.