Energy homeostasis in living organisms can be defined through the principle of conservation of matter: "Nothing is lost, nothing is created, everything is transformed" (Lavoisier, Elementary Treatise on Chemistry, 1789). The mission of the METABOL'IC analysis platform is to provide scientists with a comprehensive characterization of the components of energy homeostasis, particularly the analysis of metabolism from cell to small animal.
To date, we are equipped with an indirect calorimetry cage module (Promethion-Core©), which allows quantification of the different components of energy balance and related behaviors, as well as a body composition analyzer (Brucker MRI©), a SeaHorse XF Pro system (Agilent©), and a Cytation 1 for normalization, which enable characterization of the bioenergetic profile of cells and cellular phenotyping.
Our tools contribute to the understanding of the pathophysiology of metabolic diseases (diabetes, metabolic syndrome, eating disorders, cancer, etc.).
The scientific committee
METABOL’IC is placed under the responsibility of:
- Raphaël Denis, Research Engineer Université Paris Cité
with scientific advisers:
- Catherine Postic, directrice de recherche CNRS - équipe Signalisation du glucose et de l’insuline, et glucotoxicité
- Clotilde Alves-Guerra, Chargée de recherche CNRS - équipe Mitochondries, bioénergétique, métabolisme et signalisation
- Clotilde Cadart, Chargée de recherche - équipe Biologie quantitative de la cellule et de l'embryon
Team members
Services
The METABOL’IC platform is open to the entire public and private scientific community of Île-de-France, as well as at the national and international levels.
The mission of the METABOL'IC analysis platform is to provide scientists with a comprehensive characterization of the components of energy homeostasis in animals and cells.
- Recording of indirect calorimetry (Promethion Core©)
- Analysis and interpretation of indirect calorimetry data
- Body composition analysis in animals
- Animal housing, longitudinal studies, and monitoring (body composition, weight, food intake, temperature monitoring, diet tracking, calorimetry)
- Measurement of body temperature using BodyCAP© implants
- Measurement of surface temperature in animals using a thermal camera (Testo 883©)
- Exploration of cellular metabolism using a Seahorse Pro system (96 wells) coupled with a Cytation 1 system for well normalization. The system is located in an L2 zone.
- This includes calorimetric analysis for 8 mice.
- This corresponds to the analysis and interpretation of data from the Metabol’IC platform; the cost may vary depending on the complexity of the protocol.
- Analysis performed on data collected on a platform external to the Metabol’IC platform; the cost may vary depending on the complexity of the protocol.
- The calorimetric study requires three MRN analyses for individualization, at the entry and exit of the platform.
- This service includes two body composition analyses, weight monitoring, and diet monitoring.
- This service is performed by a platform agent, reception of samples on a plate provided by the requester, setup and reading, transmission of a report and data (excluding interpretation).
- This training includes software presentation, online resources, use of the Seahorse, data transmission (excluding interpretation), and the openIris reservation system.
- Excluding consumables
- Requires training
* Accommodation pricing.
** the exact cost will be reviewed and recalculated with the valorization and legal services.
The prices presented are indicative and can be adapted according to the number of services.
Satisfaction / Complaints
- Satisfaction Questionnaire
- For complaints, contact us
The platform services are subject to the acceptance of the conditions listed in the platform charter ("PFIC METABOL’IC Charter.pdf") and after the project has been discussed with those in charge of the platform, in order to guarantee its feasibility in accordance with the availability, the capacity of the platform and the health and ethical regulations.
The METABOL'IC platform team is available to exchange views, to discuss the various aspects of the request and the scientific project, and, in accordance with the scientific user, the realization of the experimental details, the method, the analysis and the interpretation of results.
Equipments
Indirect calorimetry
The METABOL’IC platform provides users with an indirect calorimetry system (SableSys ©) for 8 mice allowing an exhaustive exploration of the metabolic phenotype of the animal. It allows simultaneous and non-invasive monitoring and recording of food and water intake, locomotor activity and energy expenditure.
This tool thus makes it possible to evaluate parameters over time such as:
- food and water intake, (qualitative and quantitative),
- oxygen consumption (O2) and carbon dioxide (CO2) production,
- the respiratory quotient,
- total energy expenditure, at rest and during exercise,
- spontaneous locomotor activity,
- the detection of Volatile Organic Compounds.
The system also allows the visualization of different components of animal behavior.
Body Composition analysis
The Metabol’IC platform provides users with:
A body mass composition analyzer (BRUKER) for mice and rats (tissues, organs or biopsies) - LF-series. It provides a simple, fast and accurate method for the measurement of fat mass, lean mass (muscle) and water content of mouse or rat.
The analysis is non-invasive, it does not require anesthesia. Fast, the scan takes less than 2 minutes. It guarantees minimal stress for the animal.
It is an indispensable tool for longitudinal studies. Particularly on animal models presenting a suspicion of metabolic alteration (variation in weight, variation in food intake, alteration in locomotor behavior and body temperature, etc.)
Expertise and support in the development of physiological exploration protocols
The METABOL’IC platform team has solid expertise in animal physiology and functional metabolic exploration in vivo. This includes the physiological regulation of metabolic processes on one hand and, on the other hand, the processes of energy metabolism dysregulation, eating behavior, and its corollaries in pathophysiological conditions such as obesity and diabetes.
The development of scientific protocols is developed in accordance with ethical and health regulations.
In addition to expertise in conducting scientific projects, we offer expertise in the analysis and interpretation of data collected from various devices and other sites.
We offer presentations on metabolism studies for your team meetings.
Metabol’IC has joined the Diabetes Institute, a consortium of clinical researchers and laboratories created under the patronage of the Université Paris Cité. Its primary goal is to create bridges and synergies within fundamental research groups and between clinical research units.
Network
Contact
Platform METABOL'IC
Institut Cochin
INSERM U1016 - CNRS UMR8104 - Université Paris Cité
