The equipments of the GENOM'IC facility

High throughput sequencers

A NextSeq™ 2000 System and a MiSeq™ from Illumina

Illumina sequencers use sequencing by synthesis (SBS) chemistry to identify the sequence of clusters (amplified fragments) directly on the sequencing medium (flow cell).

NB : the MiSeq system is not open to autonomous use. Its use by GENOM'IC depends on the genetic service (APHP), delays may vary.

 

Instrument Read length (min - max) Ouput (min - max) Number of reads (in million / min - max)
NextSeq 2000 36 - 600 bp 10 - 540 Gb  12 - 50
MiSeq 50 - 600 bp 540 Mb - 15 Gb 100 - 1,800

 

Real-time PCR devices

Four Roche LC480s (block 384 and HRM) available in room 5021 of the Faculté building, room 723 of the Roussy building and room 311 of the Méchain building.
To book, go to the Institute's booking calendar or contact us.

 

Genomic-équipements-puces à ADN

DNA chips

The facility's Affymetrix station consists of:

  • three hybridization ovens
  • three FS450 fluidics
  • a 3000 7G scanner.

Sample quality control devices

  • Two Agilent Bioanalyzer 2100 for RNA and DNA quality control
  • Two Qubits from ThermoFisher for nucleic acid determination by fluorimetry
  • A Nanodrop 8000 for nucleic acid assay

Additional devices

  • Two Covaris S220 sonicators for ultrasonic DNA fragmentation
  • Two 2720 thermal cyclers, three 9700 thermal cyclers, two ThermoFisher SimpliAmp™ thermal cyclers
  • A NetApp 100 TB storage server.

Digital PCR

Biorad's QX100™Droplet Digital™PCR system consists of:

  • a droplet generating device
  • a droplet reader device after PCR.