High throughput sequencers
A NextSeq™ 500 System and a MiSeq™ from Illumina
Illumina sequencers use sequencing by synthesis (SBS) chemistry to identify the sequence of clusters (amplified fragments) directly on the sequencing medium (flow cell).
The NextSeq 500 System is a benchtop sequencer capable of generating 20 to 120 Gb of data in a single run, providing flexibility across a wide range of applications. Read lengths range from 75 to 300 bases.
The MiSeq System is a benchtop sequencer for targeted sequencing, small genome, metagenomics and other applications. It can generate between 0.5 and 15 Gb of data per run. Read lengths range from 36 to 600 bases.
Real-time PCR devices
Four Roche LC480s (block 384 and HRM) available in room 5021 of the Faculté building, room 723 of the Roussy building and room 311 of the Méchain building.
To book, go to the Institute's booking calendar or contact us.
DNA chips
The facility's Affymetrix station consists of:
- three hybridization ovens
- three FS450 fluidics
- a 3000 7G scanner.
Sample quality control devices
- Two Agilent Bioanalyzer 2100 for RNA and DNA quality control
- Two Qubits from ThermoFisher for nucleic acid determination by fluorimetry
- A Nanodrop 8000 for nucleic acid assay
Additional devices
- Two Covaris S220 sonicators for ultrasonic DNA fragmentation
- Two 2720 thermal cyclers, three 9700 thermal cyclers, two ThermoFisher SimpliAmp™ thermal cyclers
- A NetApp 100 TB storage server.
Digital PCR
Biorad's QX100™Droplet Digital™PCR system consists of:
- a droplet generating device
- a droplet reader device after PCR.
