Description of the Single Cell service
The GENOM'IC core facility offers expert support for your single-cell transcriptomics projects, using cutting-edge technologies developed by 10X Genomics.
We are currently one of the only platforms in the Paris region to offer personalised support at every stage of the workflow, from feasibility assessment to the production of usable data.
We support a wide range of 10X protocols, including:
🔹 3' scRNAseq (+/- surface proteins)
🔹 5' scRNAseq (+/- VDJ ou +/- surface proteins)
🔹 Multiome : expression + chromatin accessibility (ATAC) (nuclei only)
🔹 Flex GEM-X (+/- surface proteins) : new generation of kits for fixed or FFPE samples (human and mouse only)
🔹 Other specific protocols upon request
Before submitting a project, a preparatory meeting with the team is mandatory.
As each single cell project is unique, we help you define the optimal conditions:
- Sample type and upstream preparation
- Protocol adapted to scientific objectives
- Number of cells, sequencing depth, desired analyses
We are available at every stage of the project to answer your technical questions and advise you. Contact us at the earliest stages of your project to discuss it.
| Application | Starting input |
| 3', 5' or Multiome | 20 à 30 000 cells or nuclei |
| Flex |
300 000 cells minimum 500 000 nuclei minimum |
