Atypical landscape of transposable elements in the large genome of Aedes aegypti

Josquin Daron (Institut Pasteur, Paris)

15 February 2024


Pratical info

12:00 - 13:00
Conference room Rosalind Franklin

Transposable elements (TEs) contribute significantly to variation in genome size among eucaryote species, but the factors influencing TE accumulation and diversification are only partially understood. Most of our current knowledge about TE organization, dynamics and evolution derives from investigations in model organisms with a relatively small genome size such as Drosophila melanogaster or Arabidopsis thaliana. Whether the observed patterns hold true in larger genomes remains to be determined. The Diptera order is an ideal taxon to address this question, because it includes a forty-year model of TE biology (D. melanogaster) as well as mosquito species with significantly larger genomes. We used a comparative genomics approach to characterize the genomic forces that have shaped the TE content of the D. melanogaster genome (180 Mb), the Anopheles coluzzii genome (300 Mb) and the Aedes aegypti genome (1.5Gb) . Leveraging a newly developed high-quality TE library for Ae. aegypti, our results reveal a contrasted pattern of TE organization in Ae. aegypti compared to D. melanogaster and An. coluzzii. Combined genomics and population genomics approaches suggest that the high TE content observed in Ae. aegypti is associated to both a high rate of recent TE transposition and a low rate of TE elimination. Together, our results indicate that TE organization and evolutionary dynamics in the large genome of Ae. aegypti are distinct from those of other Diptera with smaller genomes.

Invited by Suzanne Faure-Dupuy, Alberto De la Iglesia and Hugo Barreto, as part of the Post-doc seminar series.

Selected publications

  • Margaux J. M. Lefebvre, Josquin Daron, Eric Legrand, Michael C. Fontaine, Virginie Rougeron, Franck Prugnolle. Population genomic evidence of adaptive response during the invasion history of Plasmodium falciparum in the Americas. bioRxiv 2022.10.30.514183; doi: 10.1101/2022.10.30.514183
  • Picard MAL, Leblay F, Cassan C, Willemsen A, Daron J, Bauffe F, Decourcelle M, Demange A, Bravo IG. Transcriptomic, proteomic, and functional consequences of codon usage bias in human cells during heterologous gene expression. Protein Sci. 2023 Mar;32(3):e4576. doi: 10.1002/pro.4576.
  • Daron J, Boissière A, Boundenga L, Ngoubangoye B, Houze S, Arnathau C, Sidobre C, Trape JF, Durand P, Renaud F, Fontaine MC, Prugnolle F, Rougeron V. Population genomic evidence of Plasmodium vivax Southeast Asian origin. Sci Adv. 2021 Apr 28;7(18):eabc3713. doi: 10.1126/sciadv.abc3713.
  • Lai Y, Lu XM, Daron J, Pan S, Wang J, Wang W, Tsuchiya T, Holub E, McDowell JM, Slotkin RK, Le Roch KG, Eulgem T. The Arabidopsis PHD-finger protein EDM2 has multiple roles in balancing NLR immune receptor gene expression. PLoS Genet. 2020 Sep 14;16(9):e1008993. doi: 10.1371/journal.pgen.1008993.
  • Daron J, Slotkin RK. EpiTEome: Simultaneous detection of transposable element insertion sites and their DNA methylation levels. Genome Biol: 2017;18(91) doi:10.1186/s13059-017-1232-0