Neutrophil and Macrophage Heterogeneity in Prostate Cancer

Sandrine Henri (Pathophysiology of lung infections team in Tours)

26 March 2026

Seminar

Pratical info

12:00 - 13:00

Conference room Rosalind Franklin

research professional

Reduced mobility access

The immune landscape of healthy prostate and its alterations during prostate cancer (PCa) progression remain poorly characterized. Using scRNA-sequencing and multiparametric flow-cytometry analysis, we comprehensively characterized immune cells in wild-type and PTEN(i)pe−/− mouse prostates, a model that closely recapitulates human PCa. PCa in PTEN(i)pe−/− is marked by the recruitment of tumor-associated neutrophils (TANs), which represent the dominant immune cell population and resolved into eight distinct states, Trem2+ tumor-associated-macrophages (TAMs), and exhausted CD8+ T cells. Trem2+ TAMs differ from the three main resident macrophage populations in the healthy prostate, exhibiting a strong metabolic and immunosuppressive signature, likely driven by the MIF/HIF1A-signaling axis. This study provides the first detailed characterization of immune cells in the healthy mouse prostate and reveals changes in the immune landscape associated with prostate cancer progression.

Sandrine Henri is invited by Julie Helft.

Selected publications

Pervizou D, De Chiara J, Spinelli L, Nestor-Martin M, Chasson L, Len-Tayon K, Yanushko D, Fiore F, Bajénoff M, Malissen B, Metzger D, Laverny G, Henri S. Characterization of the immune landscape in healthy mouse prostate and during prostate cancer progression. Oncoimmunology. 2025 Dec;14(1):2562220. doi: 10.1080/2162402X.2025.2562220. Epub 2025 Sep 26. PMID: 40999879; PMCID: PMC12477881.

Lanza JS, Hammoudi A, De Chiara J, Surenaud M, Kembou A, Esposito M, Zurawski S, Zurawski G, Centlivre M, Malissen B, Godot V, Lévy Y, Henri S, Cardinaud S. Targeting Langerhans cells via skin delivery of HIV Envelope enhances the antibody response to vaccination. NPJ Vaccines. 2025 Jul 25;10(1):170. doi: 10.1038/s41541-025-01214-w. PMID: 40715153; PMCID: PMC12297313.

Henri S, Chesnay A, Ait Belkacem I, Gonzalez L, Barsac E, Malergue F, Desoubeaux G. Fungal infection does not impair flow cytometry signals induced by nCD64 and mCD169 biomarkers during co-infection with bacteria and viruses. J Mycol Med. 2025 Sep;35(3):101562. doi: 10.1016/j.mycmed.2025.101562. Epub 2025 Jun 21. PMID: 40609155.

Strandt H, Voluzan O, Niedermair T, Ritter U, Thalhamer J, Malissen B, Stoecklinger A, Henri S. Macrophages and Fibroblasts Differentially Contribute to Tattoo Stability. Dermatology. 2021;237(2):296-302. doi: 10.1159/000506540. Epub 2020 Apr 28. PMID: 32344413.

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