Generation of transgene-free hematopoietic stem cells from human induced pluripotent stem cells

Thierry Jaffredo (Institute of Biology Paris-Seine)

05 octobre 2023

Jaffredo THierry

Infos pratiques

12h00 - 13h00
Salle Rosalind Franklin
Professionnel de recherche
Accès mobilité réduite

Hematopoietic stem cells (HSCs) are the rare cells responsible for the lifelong curative effects of hematopoietic cell transplantation. The demand for clinical-grade HSCs has increased significantly in recent decades, leading to major difficulties in treating patients. A promising but not yet achieved goal is the generation of HSCs from pluripotent stem cells. Here, we have obtained vector- and stroma-free transplantable HSCs by differentiating human induced pluripotent stem cells (hiPSCs) using an original one-step culture system. After injection into immunocompromised mice, cells derived from hiPSCs settle in the bone marrow and form a robust multilineage, hematopoietic population that can be serially transplanted. Single cell RNA sequencing shows that this repopulating activity is due to a hematopoietic population that is transcriptionally similar to human embryonic aorta-derived HSCs. Overall, our results demonstrate the generation of HSCs from hiPSC and will help identify key regulators of HSC production during human ontogeny.

Thierry Jaffredo is invited by Pascal Maire.