The function of many organs, including skeletal muscle, depends on its three-dimensional structure. Muscle regeneration therefore requires not only reestablishment of myofibers, but restoration of tissue architecture. Resident muscle stem cells (SCs) are essential for regeneration, but how SCs regenerate muscle architecture is largely unknown. Using genetic labeling of SCs and whole mount imaging to reconstruct in three dimensions muscle regeneration, we address this problem. I will present unpublished data revealing new insights into the cellular events of regeneration.

Gabrielle Kardon is invited by Pascal Maire.