4 nanoLC-MSMS systems
160 projects / year
7 expert members
61 publications (2018-2022)
98.5% of satisfied users on the last five years
Members
Scientific committee
- Dr. Pierre Sohier: Cutaneous Biology team (Sélim Aractingi) and Anatomo-Pathologist doctor at Cochin Hospital
- Dr. Ismaël Boussaïd: Normal and Pathological Hematopoiesis team (Michaëla Fontenay & Didier Bouscary) and pharmacist biologist at Cochin Hospital
- Dr. Frédérique Verdier: Team Biology of Plasmodium Transmission (Catherine Lavazec)
- Dr. Gwladys Bertin: Institute of Research for Development (IRD)
Services
PROTEOM'IC has materials and expertise to respond to a wide range of proteomics requests:
Global comparative quantitative analyzes of proteome / secretomes (relative or absolute quantification, Label Free, SILAC, ITRAQ, TMT)
Identification and comparative quantification of protein partners (AP-MS / CO-IP / Bio-ID)
Global Comparative phosphoproteomic analyzes
Global Comparative analyzes of acetylations, N-glycosylations or other post-transduction changes, on request
Analysis of FFPE sections (fixed biological material and included in paraffin)
Identifications and characterization of proteins (from freezing fragments or in solution)
1D / 2D Western Blots
Functional analyzes (proteomics, transcriptomics, metabolomics, integrative analyzes, training, provision of an analysis post)
Equipment
The core facility is equipped with various equipment intended for the preparation of samples for their analysis by mass spectrometry by different analyzers and ionization modes: 2 timsTOF Pro mass spectrometers and 2 Orbitrap type mass spectrometers (Fusion and Qexactive plus). It has a clean room equipped with a horizontal laminar flow hood for sample preparation and high-performance IT resources. It also has direct access to other equipment located on Institut Cochin site: AKTA FPLC and AKTA Micro chromatography systems, Typhoon fluorescence scanner, ultracentrifuges, lyophilizer.
Prices
The prices indicated concern exclusively the academic research teams of Université Paris Cité, Inserm and CNRS for the left column as well as the academic research teams from other EPST or EPIC for the right column.
Requests relating to private research teams will be the subject of a quote.
Current services
|
Current services |
Université Paris Cité, Inserm or CNRS |
Other EPST or EPIC |
|
Identification of protein partners * |
120 € / sample |
200 € / sample |
|
Label-Free * |
450 € / sample |
550 € / sample |
|
Label-Free and absolute quantification * |
450 € / sample |
550 € / sample |
|
Label-Free from FFPE sections * |
500 € / sample |
600 € / sample |
|
Label-Free from plasma (depletion kit included) * |
550 € / sample |
650 € / sample |
|
Phosphoproteomics * |
300 € / sample |
350 € / sample |
|
1D analysis (or of spot 2D) |
40 € / sample |
75 € / sample |
|
Analysis of a protein purified in solution |
80 € / sample |
150 € / sample |
|
Synthetic functional Analysis |
220 € / project |
310 € / project |
|
Training on functional analysis softwares |
80 € / hour |
120 € / hour |
|
Provision of functional analysis software on the platform |
530 € / year |
670 € / year |
* * Including the preparation and statistical analysis. Price per sample and replicat. NB: minimum n = 4 biological replicates with 1 replicat offered from n = 5 biological replicats.
Services on request (and with quote)
- Mini 2D gel, mini 2D gel + transfer to membrane or 2D-western blot
- ITRAQ or TMT
- Enrichment of modified peptides
- Micro-flow preparative liquid chromatography
Training
Publications
Contacts
by email:
by phone:
+33. 1.40.51.64.99 : Emilie-Fleur Gautier
+33. 1.40.51.64.98 : Cédric Broussard / Marjorie Leduc / Virginie Salnot
+33. 1.40.51.64.17 : Johanna Bruce
+33. 1.40.51.64.35 : Guilhem Clary
+33. 1.40.51.64.36 : Morgane Le Gall
by postal mail:
Institut Cochin - 22 rue Méchain - Bâtiment Méchain - RDC - 75014 Paris
Funding
