Biomedical research institute


    Selected research publications of the members of the team since ten years

    1. Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils.
      Ohayon D, De Chiara A, Dang PM, Thieblemont N, Chatfield S, Marzaioli V, Burgener SS, Mocek J, Candalh C, Pintard C, Tacnet-Delorme P, Renault G, Lagoutte I, Favier M, Walker F, Hurtado-Nedelec M, Desplancq D, Weiss E, Benarafa C, Housset D, Marie JC, Frachet P, El-Benna J, Witko-Sarsat V.
      J Exp Med. 2019 Sep 6. pii: jem.20180371. doi: 10.1084/jem.20180371.
    2. Proteomic analysis of neutrophils in ANCA-associated vasculitis reveals a dysregulation in proteinase 3-associated proteins such as annexin-A1 involved in apoptotic cell clearance.
      Everts-Graber J, Martin KR, Thieblemont N, Mocek J, Roccabianca A, Chafey P, Le Gall M, Tacnet-Delorme P, Reutelingsperger CP, Naccache JM, Bonnotte B, Karras A, Puéchal X, Guillevin L, Terrier B, Frachet P, Perretti M, Mouthon L, Witko-Sarsat V.
      Kidney Int. 2019;96:397-408. doi: 10.1016/j.kint.2019.02.017.
    3. Inflammasome activation: Neutrophils go their own way
      Tourneur L, Witko-Sarsat V.
      J Leukoc Biol. 2019 Mar;105:433-436.
    4. The classical NLRP3 inflammasome controls FADD unconventional secretion through microvesicle shedding.
      Mouasni S, Gonzalez V, Schmitt A, Bennana E, Guillonneau F, Mistou S, Avouac J, Ea HK, Devauchelle V, Gottenberg JE, Chiocchia G, Tourneur L.
      Cell Death Dis. 2019; 10:190.
    5. Expanding Neutrophil Horizons: New Concepts in Inflammation.
      Chatfield SM, Thieblemont N, Witko-Sarsat V.
      J Innate Immun. 2018;10(5-6):422-431. doi: 10.1159/000493101.
    6. Regulation of macrophage activation by proteins expressed on apoptotic neutrophils: Subversion towards autoimmunity by proteinase 3.
      Thieblemont N, Witko-Sarsat V, Ariel A.
      Eur J Clin Invest. 2018 Nov;48 Suppl 2:e12990. Review.
    7. FADD at the Crossroads between Cancer and Inflammation
      Mouasni S, Tourneur L
      Trends Immunol. 2018; 39:1036-1053.
    8. Proteinase 3 Interferes With C1q-Mediated Clearance of Apoptotic Cells.
      Tacnet-Delorme P, Gabillet J, Chatfield S, Thieblemont N, Frachet P, Witko-Sarsat V.
      Front Immunol. 2018 Apr 25;9:818. doi: 10.3389/fimmu.2018.00818.
    9. mTOR pathway is activated in endothelial cells from patients with Takayasu arteritis and is modulated by serum immunoglobulin G.
      Hadjadj J, Canaud G, Mirault T, Samson M, Bruneval P, Régent A, Goulvestre C, Witko-Sarsat V, Costedoat-Chalumeau N, Guillevin L, Mouthon L, Terrier B; French Vasculitis Study Group.
      Rheumatology (Oxford). 2018 Jun 1;57(6):1011-1020. doi: 10.1093/rheumatology/key017.
    10. Transgenic mice expressing human proteinase 3 exhibit sustained neutrophil-associated peritonitis.
      Martin K, Pederzoli-Ribeil M, Pacreau E, Burgener S, Dahdah A, Candalh C, Foretz M, Mouthon L, Lucas B, Thieblemont N, Benarafa C, Launay P, Witko-Sarsat V. Transgenic mice expressing human proteinase 3 exhibit sustained neutrophil-associated peritonitis. 
      J Immunol 2017 Dec 1;199(11):3914-3924. doi: 10.4049/jimmunol..
    11. Scleroderma Peripheral B Lymphocytes Secrete Interleukin-6 and Transforming Growth Factor β and Activate Fibroblasts.
      Dumoitier N, Chaigne B, Régent A, Lofek S, Mhibik M, Dorfmüller P, Terrier B, London J, Bérezné A, Tamas N, Varin-Blank N, Mouthon L.
      Arthritis Rheumatol. 2017 May;69(5):1078-1089. doi: 10.1002/art.40016.
    12. Harnessing Neutrophil Survival Mechanisms during Chronic Infection by Pseudomonas aeruginosa: Novel Therapeutic Targets to Dampen Inflammation in Cystic Fibrosis.
      Marteyn BS, Burgel PR, Meijer L, Witko-Sarsat V.
      Front Cell Infect Microbiol. 2017 Jun 30;7:243. doi: 10.3389/fcimb.2017.00243. Review.
    13. Proteinase 3: the odd one out that became an autoantigen.
      Martin KR, Witko-Sarsat V.
      J Leukoc Biol. 2017 May 25. pii: jlb.3MR0217-069R. doi: 10.1189/jlb.3MR0217-069R.
    14. Granulomatosis with polyangiitis (Wegener granulomatosis): A proteinase-3 driven disease?
      Witko-Sarsat V, Thieblemont N.
      Joint Bone Spine. 2017 May 8. pii: S1297-319X(17)30089-1. doi: 10.1016/j.jbspin.2017.05.004.
    15. Relevance of diagnostic investigations in patients with uveitis: Retrospective cohort study on 300 patients.
      Hadjadj J, Dechartres A, Chapron T, Assala M, Salah S, Dunogué B, Musset L, Baudin B, Groh M, Blanche P, Mouthon L, Monnet D, Le Jeunne C, Brézin A, Terrier B.
      Autoimmun Rev. 2017 ;16(5):504-511. doi: 10.1016/j.autrev.2017.03.006. Review.
    16. Molecular analysis of vascular smooth muscle cells from patients with giant cell arteritis: Targeting endothelin-1 receptor to control proliferation.
      Régent A, Ly KH, Groh M, Khifer C, Lofek S, Clary G, Chafey P, Baud V, Broussard C, Federici C, Labrousse F, Mesturoux L, Le Jeunne C, Vidal E, Brezin A, Witko-Sarsat V, Guillevin L, Mouthon L.
      Autoimmun Rev. 2017 Apr;16(4):398-406. doi: 10.1016/j.autrev.2017.02.006. Review.
    17. Anti-endothelial cell antibodies in vasculitis: A systematic review.
      Legendre P, Régent A, Thiebault M, Mouthon L.
      Autoimmun Rev. 2017 Feb;16(2):146-153. doi: 10.1016/j.autrev.2016.12.012. Review.
    18. Molecular analysis of vascular smooth muscle cells from patients with giant cell arteritis: Targeting endothelin-1 receptor to control proliferation.
      Régent A, Ly KH, Groh M, Khifer C, Lofek S, Clary G, Chafey P, Baud V, Broussard C, Federici C, Labrousse F, Mesturoux L, Le Jeunne C, Vidal E, Brezin A, Witko-Sarsat V, Guillevin L, Mouthon L.
      Autoimmun Rev. 2017 Apr;16(4):398-406. doi: 10.1016/j.autrev.2017.02.006. Review.
    19. Anti-endothelial cell antibodies in vasculitis: A systematic review.
      Legendre P, Régent A, Thiebault M, Mouthon L.
      Autoimmun Rev  2017, 16:146-153.
    20. Cytoplasmic proliferating cell nuclear antigen connects glycolysis and cell survival in acute myeloid leukemia.
      Ohayon D, De Chiara A, Chapuis N, Candalh C, Mocek J, Ribeil JA, Haddaoui L, Ifrah N, Hermine O, Bouillaud F, Frachet P, Bouscary D, Witko-Sarsat V.
      Sci Rep. 2016 Oct 19;6:35561. doi: 10.1038/srep35561.
    21. Proliferating cell nuclear antigen in neutrophil fate.
      Witko-Sarsat V, Ohayon D.
      Immunol Rev. 2016 Sep;273(1):344-56. doi: 10.1111/imr.12449. Review.
    22. Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
      Martin KR, Kantari-Mimoun C, Yin M, Pederzoli-Ribeil M, Angelot-Delettre F, Ceroi A, Grauffel C, Benhamou M, Reuter N, Saas P, Frachet P, Boulanger CM, Witko-Sarsat V.
      J Biol Chem. 2016 May 13;291(20):10476-89. doi: 10.1074/jbc.M115.698639.
    23. Proteomic analysis of vascular smooth muscle cells in physiological condition and in pulmonary arterial hypertension: Toward contractile versus synthetic phenotypes.
      Régent A, Ly KH, Lofek S, Clary G, Tamby M, Tamas N, Federici C, Broussard C, Chafey P, Liaudet-Coopman E, Humbert M, Perros F, Mouthon L.
      Proteomics. 2016 Oct;16(20):2637-2649. doi: 10.1002/pmic.201500006.
    24. Neutrophil-Expressed p21/waf1 Favors Inflammation Resolution in Pseudomonas aeruginosa Infection.
      Martin C, Ohayon D, Alkan M, Mocek J, Pederzoli-Ribeil M, Candalh C, Thevenot G, Millet A, Tamassia N, Cassatella MA, Thieblemont N, Burgel PR, Witko-Sarsat V.
      Am J Respir Cell Mol Biol. 2016 May;54(5):740-50. doi: 10.1165/rcmb.2015-0047OC.
    25. Anoxia and glucose supplementation preserve neutrophil viability and function.
      Monceaux V, Chiche-Lapierre C, Chaput C, Witko-Sarsat V, Prevost MC, Taylor CT, Ungeheuer MN, Sansonetti PJ, Marteyn BS.
      Blood. 2016 Aug 18;128(7):993-1002. doi: 10.1182/blood-2015-11-680918.
    26. Modulating Innate and Adaptive Immunity by (R)-Roscovitine: Potential Therapeutic Opportunity in Cystic Fibrosis.
      Meijer L, Nelson DJ, Riazanski V, Gabdoulkhakova AG, Hery-Arnaud G, Le Berre R, Loaëc N, Oumata N, Galons H, Nowak E, Gueganton L, Dorothée G, Prochazkova M, Hall B, Kulkarni AB, Gray RD, Rossi AG, Witko-Sarsat V, Norez C, Becq F, Ravel D, Mottier D, Rault G.
      J Innate Immun. 2016;8(4):330-49. doi: 10.1159/000444256. Epub 2016 Mar 18. Review.
    27. From Starfish Oocytes to Inflammation: The Unforeseeable Destiny of Roscovitine in Cystic Fibrosis.
      Witko-Sarsat V.
      J Innate Immun. 2016;8(4):327-9. doi: 10.1159/000445967.
    28. Dividing the Janus vasculitis? Pathophysiology of eosinophilic granulomatosis with polyangitis.
      Chaigne B, Terrier B, Thieblemont N, Witko-Sarsat V, Mouthon L.
      Autoimmun Rev. 2016 Feb;15(2):139-45. doi: 10.1016/j.autrev.2015.10.006.
    29. MyD88 modulates eosinophil and neutrophil recruitment as well as IL-17A production during allergic inflammation.
      Pham Van L, Germaud N, Ramadan A, Thieblemont N.
      Cell Immunol. 2016 Dec;310:116-122. doi: 10.1016/j.cellimm.2016.08.014.
    30. Human neutrophils in auto-immunity.
      Thieblemont N, Wright HL, Edwards SW, Witko-Sarsat V.
      Semin Immunol (2016), 28:159-73.
    31. Histidine Decarboxylase Deficiency Prevents Autoimmune Diabetes in NOD Mice.
      Alkan M, Machavoine F, Rignault R, Dam J, Dy M, Thieblemont N.
      J Diabetes Res. 2015;2015:965056. doi: 10.1155/2015/965056. Epub 2015 May 18.
    32. Implication of B lymphocytes in the pathogenesis of ANCA-associated vasculitides.
      Dumoitier N, Terrier B, London J, Lofek S, Mouthon L.
      Autoimmun Rev. 2015 Nov;14(11):996-1004. doi: 10.1016/j.autrev.2015.06.008. Review.
    33. Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.
      Millet A, Martin KR, Bonnefoy F, Saas P, Mocek J, Alkan M, Terrier B, Kerstein A, Tamassia N, Satyanarayanan SK, Ariel A, Ribeil JA, Guillevin L, Cassatella MA, Mueller A, Thieblemont N, Lamprecht P, Mouthon L, Perruche S, Witko-Sarsat V.
      J Clin Invest. 2015 Nov 2;125(11):4107-21. doi: 10.1172/JCI78182. Epub 2015 Oct 5.
    34. Identification of target antigens of anti-endothelial cell antibodies in patients with anti-neutrophil cytoplasmic antibody-associated vasculitides: a proteomic approach.
      Régent A, Lofek S, Dib H, Bussone G, Tamas N, Federici C, Broussard C, Guillevin L, Mouthon L. Clin Immunol (2014), 153(1):123-35.
    35. Neurotrophins are expressed in giant cell arteritis lesions and may contribute to vascular remodeling.
      Ly KH, Régent A, Molina E, Saada S, Sindou P, Le-Jeunne C, Brézin A, Witko-Sarsat V, Labrousse F, Robert PY, Bertin P, Bourges JL, Fauchais AL, Vidal E, Mouthon L, Jauberteau MO.
      Arthritis Res Ther. 2014 Nov 24;16(6):487. doi: 10.1186/s13075-014-0487-z.
    36. Conventional but not plasmacytoid dendritic cells foster the systemic virus-induced type I IFN response needed for efficient CD8 T cell priming.
      Hervas-Stubbs S, Riezu-Boj JI, Mancheño U, Rueda P, Lopez L, Alignani D, Rodríguez-García E, Thieblemont N, Leclerc C.
      J Immunol. 2014 Aug 1;193(3):1151-61. doi: 10.4049/jimmunol.1301440.
    37. Intravenous immunoglobulin-induced IL-33 is insufficient to mediate basophil expansion in autoimmune patients.
      Sharma M, Schoindre Y, Hegde P, Saha C, Maddur MS, Stephen-Victor E, Gilardin L, Lecerf M, Bruneval P, Mouthon L, Benveniste O, Kaveri SV, Bayry J.
      Sci Rep. 2014 Jul 11;4:5672. doi: 10.1038/srep05672.
    38. CD21-/lo marginal zone-like B cells in HCV-related mixed cryoglobulinemia vasculitis highly express FCRL5 protein and are killed by anti-FCRL5 immunotoxins.
      Terrier B, Nagata S, Ise T, Rosenzwajg M, Pastan I, Klatzmann D, Saadoun D, Cacoub P.
      Arthritis Rheumatol (2014), 66(2):433-43
    39. Characterization of cytosolic proliferating cell nuclear antigen (PCNA) in neutrophils: antiapoptotic role of the monomer.
      De Chiara A, Pederzoli-Ribeil M, Mocek J, Candalh C, Mayeux P, Millet A, Witko-Sarsat V.
      J Leukoc Biol. 2013 Oct;94(4):723-31. doi: 10.1189/jlb.1212637. Epub 2013 Jul 3.
    40. Antineutrophil cytoplasmic antibody-associated vasculitides: is it time to split up the group?
      Millet A, Pederzoli-Ribeil M, Guillevin L, Witko-Sarsat V, Mouthon L.
      Ann Rheum Dis. 2013 Aug;72(8):1273-9. doi: 10.1136/annrheumdis-2013-203255. Review.
    41. Neutrophils in the innate immunity conundrum of cystic fibrosis: a CFTR-related matter?
      Witko-Sarsat V.
      J Innate Immun. 2013;5(3):195-6. doi: 10.1159/000350215.
    42. Autophagy and innate immunity.
      Witko-Sarsat V, Codogno P.
      J Innate Immun. 2013;5(5):425-6. doi: 10.1159/000353153.
    43. Activation of basophils by the double-stranded RNA poly(A:U) exacerbates allergic inflammation.
      Ramadan A, Pham Van L, Machavoine F, Dietrich C, Alkan M, Karasuyama H, Schneider E, Dy M, Thieblemont N.
      Allergy. 2013 Jun;68(6):732-8. doi: 10.1111/all.12151.
    44. Cryoglobulinemia vasculitis: an update.
      Terrier B, Cacoub P.
      Curr Opin Rheumatol. 2013 Jan;25(1):10-8. doi: 10.1097/BOR.0b013e32835b15f7.
    45. Proteomes of umbilical vein and microvascular endothelial cells reflect distinct biological properties and influence immune recognition.
      Dib H, Chafey P, Clary G, Federici C, Le Gall M, Dwyer J, Gavard J, Tamas N, Bussone G, Broussard C, Camoin L, Witko-Sarsat V, Tamby MC, Mouthon L.
      Proteomics. 2012 Aug;12(15-16):2547-55. doi: 10.1002/pmic.201200060.
    46. Nuclear-to-cytoplasmic relocalization of the proliferating cell nuclear antigen (PCNA) during differentiation involves a chromosome region maintenance 1 (CRM1)-dependent export and is a prerequisite for PCNA antiapoptotic activity in mature neutrophils.
      Bouayad D, Pederzoli-Ribeil M, Mocek J, Candalh C, Arlet JB, Hermine O, Reuter N, Davezac N, Witko-Sarsat V.
      J Biol Chem. 2012 Sep 28;287(40):33812-25.
    47. Proteinase 3, the autoantigen in granulomatosis with polyangiitis, associates with calreticulin on apoptotic neutrophils, impairs macrophage phagocytosis, and promotes inflammation.
      Gabillet J, Millet A, Pederzoli-Ribeil M, Tacnet-Delorme P, Guillevin L, Mouthon L, Frachet P, Witko-Sarsat V.
      J Immunol. 2012 Sep 1;189(5):2574-83. doi: 10.4049/jimmunol.1200600.
    48. Restoration of regulatory and effector T cell balance and B cell homeostasis in systemic lupus erythematosus patients through vitamin D supplementation.
      Terrier B, Derian N, Schoindre Y, Chaara W, Geri G, Zahr N, Mariampillai K, Rosenzwajg M, Carpentier W, Musset L, Piette JC, Six A, Klatzmann D, Saadoun D, Patrice C, Costedoat-Chalumeau N.
      Arthritis Res Ther. 2012 Oct 17;14(5):R221. doi: 10.1186/ar4060.
    49. IgG from patients with pulmonary arterial hypertension and/or systemic sclerosis binds to vascular smooth muscle cells and induces cell contraction.
      Bussone G, Tamby MC, Calzas C, Kherbeck N, Sahbatou Y, Sanson C, Ghazal K, Dib H, Weksler BB, Broussard C, Verrecchia F, Yaici A, Witko-Sarsat V, Simonneau G, Guillevin L, Humbert M, Mouthon L.
      Ann Rheum Dis. 2012 Apr;71(4):596-605. doi: 10.1136/annrheumdis-2011-200195.
    50. Targets of anti-endothelial cell antibodies in pulmonary hypertension and scleroderma.
      Dib H, Tamby MC, Bussone G, Regent A, Berezné A, Lafine C, Broussard C, Simonneau G, Guillevin L, Witko-Sarsat V, Humbert M, Mouthon L.
      Eur Respir J. 2012 Jun;39(6):1405-14. doi: 10.1183/09031936.00181410.
    51. Interleukin 21 correlates with T cell and B cell subset alterations in systemic lupus erythematosus.
      Terrier B, Costedoat-Chalumeau N, Garrido M, Geri G, Rosenzwajg M, Musset L, Klatzmann D, Saadoun D, Cacoub P.
      J Rheumatol. 2012 Sep;39(9):1819-28. doi: 10.3899/jrheum.120468.
    52. Interleukin-21 modulates Th1 and Th17 responses in giant cell arteritis.
      Terrier B, Geri G, Chaara W, Allenbach Y, Rosenzwajg M, Costedoat-Chalumeau N, Fouret P, Musset L, Benveniste O, Six A, Klatzmann D, Saadoun D, Cacoub P.
      Arthritis Rheum. 2012 Jun;64(6):2001-11. doi: 10.1002/art.34327.
    53. Targeting cytosolic proliferating cell nuclear antigen in neutrophil-dominated inflammation.
      Chiara AD, Pederzoli-Ribeil M, Burgel PR, Danel C, Witko-Sarsat V.
      Front Immunol. 2012 Oct 9;3:311. doi: 10.3389/fimmu.2012.00311.
    54. FADD protein release mirrors the development and aggressiveness of human non-small cell lung cancer.
      Cimino Y, Costes A, Damotte D, Validire P, Mistou S, Cagnard N, Alifano M, Régnard JF, Chiocchia G, Sautès-Fridman C, Tourneur L.
      Br J Cancer. 2012 Jun 5;106(12):1989-96. doi: 10.1038/bjc.2012.196.
    55. Fas-associated death domain protein and adenosine partnership: FAD in RA.
      Vilmont V, Tourneur L, Chiocchia G.
      Rheumatology (Oxford). 2012 Jun;51(6):964-75.
    56. Regulating neutrophil apoptosis: new players enter the game.
      Witko-Sarsat V, Pederzoli-Ribeil M, Hirsch E, Sozzani S, Cassatella MA.
      Trends Immunol. 2011 Mar;32(3):117-24. doi: 10.1016/ Review.
    57. Molecular analysis of the membrane insertion domain of proteinase 3, the Wegener's autoantigen, in RBL cells: implication for its pathogenic activity.
      Kantari C, Millet A, Gabillet J, Hajjar E, Broemstrup T, Pluta P, Reuter N, Witko-Sarsat V.
      J Leukoc Biol. 2011 Nov;90(5):941-50. doi: 10.1189/jlb.1210695.
    58. Proliferating cell nuclear antigen acts as a cytoplasmic platform controlling human neutrophil survival.
      Witko-Sarsat V, Mocek J, Bouayad D, Tamassia N, Ribeil JA, Candalh C, Davezac N, Reuter N, Mouthon L, Hermine O, Pederzoli-Ribeil M, Cassatella MA.
      J Exp Med. 2010 Nov 22;207(12):2631-45. doi: 10.1084/jem.20092241.
    59. Structures of human proteinase 3 and neutrophil elastase--so similar yet so different.
      Hajjar E, Broemstrup T, Kantari C, Witko-Sarsat V, Reuter N.
      FEBS J. 2010 May;277(10):2238-54.
    60. In cystic fibrosis homozygotes and heterozygotes, neutrophil apoptosis is delayed and modulated by diamide or roscovitine: evidence for an innate neutrophil disturbance.
      Moriceau S, Lenoir G, Witko-Sarsat V.
      J Innate Immun. 2010;2(3):260-6.
    61. Interaction of proteinase 3 with its associated partners: implications in the pathogenesis of Wegener's granulomatosis.
      Witko-Sarsat V, Reuter N, Mouthon L.
      Curr Opin Rheumatol. 2010 Jan;22(1):1-7.
    62. Coronin-1 is associated with neutrophil survival and is cleaved during apoptosis: potential implication in neutrophils from cystic fibrosis patients.
      Moriceau S, Kantari C, Mocek J, Davezac N, Gabillet J, Guerrera IC, Brouillard F, Tondelier D, Sermet-Gaudelus I, Danel C, Lenoir G, Daniel S, Edelman A, Witko-Sarsat V.
      J Immunol. 2009 Jun 1;182(11):7254-63.