Team: Immune Cell Signaling and Retroviral Infection

Team leader

	Clotilde Randriamampita     DR2 CNRS

 Objectives

The objectives of our team are to study issues related to the dynamics of immune cell signaling and retroviral infection.

We are studying early signaling events triggered in T lymphocytes stimulated with chemokines and want to determine how GTPases of the Rho family are regulated upon T cell activation and stimulation (lien vers JD). We also analyze the role of FoxO1, a major transcription factor which controls T cell migration and activation (lien vers MM)

Macrophages and dendritic cells are HIV-1 targets which participate to viral pathogenesis. Our objective is to characterize cellular and viral mechanisms involved in HIV-1 transfer and intercellular dissemination in target myeloid cells.

M. Mangeney	J. Delon	S. Bénichou	C. Randriamampita

Main publications

• Roux A, Bracq  L, De Muylder B, Leroy H, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G & Mangeney M. FOXO1 transcription factor plays a key role in T cell – HIV-1 interaction. PlosPathogens. Sous presse.
• Megrelis, L., El Ghoul, E., Moalli, F., Versapuech, M., Cassim, S., Ruef, N., Stein, J.V., Mangeney, M. and Delon J. (2018) Fam65b phosphorylation relieves tonic RhoA inhibition during T cell migration. Front Immunol. 9:2001

• Bracq, L., Xie, M., Lambelé, M., Vu, L.-T., Matz, J., Schmitt, A., Delon, J., Zhou, P., Randriamampita, C., Bouchet, J., and Benichou, S. (2017). T cell-macrophage fusion triggers multinucleated giant cell formation for HIV-1 spreading. J. Virol., 91, e01237-17.
• Liu, L., Wang, W., Matz, J., Ye, C., Bracq, L., Delon, J., Kimata, J., Chen, Z., Benichou, S.* and Zhou, P.* (2016) Glycosyl Phosphatidylinositol-Anchored Variable Region of Llama Heavy Chain Only Antibody JM4 Efficiently Blocks Both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1. J. Virol., 90, 10642-10659. (*equal contribution)
• Guedj, C., Abraham, N., Jullié, D., Randriamampita, C. (2016) T cell adhesion triggers an early signaling pole distal to the immune synapse. J Cell Sci. 129: 2526-2537 
• Froehlich, J., Versapuech, M., Megrelis, L., Largeteau, Q., Meunier, S., Tanchot, C., Bismuth, G., Delon, J., Mangeney, M. (2016). FAM65B controls the proliferation of transformed and primary T cells. Oncotarget. 7: 63215-63225.

Team's news

• Serge Benichou est directeur du Laboratoire International Associé (LIA VirHost) établi entre son groupe de l'Institut Cochin et l'Institut Pasteur de Shanghai.
• Accueil d'étudiants en Thèse chinois dans le cadre du programme du "Chinese Scholarship Council".
• Lauréats du Prix de l'Institut Cochin (Journées des Doctorants) : Lucie Bracq (2016), Laura Megrelis (2014), Chloé Guedj (2013).
• L'équipe participe activement au Programme Apprenti-Chercheur mis en place depuis plusieurs années à l'Institut Cochin en partenariat avec l'Arbre des connaissances
• L'équipe est membre du club cytosquelette parisien et participe activement au groupe Biosensor du GDR Imabio