Biomedical research institute


    Type IV pilus retraction enables sustained bacteremia and plays a key role in the outcome of meningococcal sepsis in a humanized mouse model.
    Barnier JP, Euphrasie D, Join-Lambert O, Audry M, Schonherr-Hellec S, Schmitt T, Bourdoulous S, Coureuil M, Nassif X, El Behi M.
    PLoS Pathog. (2021) Feb 16;17(2):e1009299.

    Meningococcal disease: a paradigm of type-IV pilus dependent pathogenesis.
    dos Santos Souza I, Maïssa N, Ziveri J, MorandPC, Coureuil M, Nassif X and Bourdoulous S
    Cell Microbiol. (2020) 22:e13185. Review.

    Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.
    Le Guennec L, Virion Z, Bouzinba-Ségard H, Robbe-Masselot C, Léonard F, Nassif X, Bourdoulous S* and Coureuil M* (*equal contribution)
    Proc Natl Acad Sci U S A. (2020) Feb 4;117(5):2606-2612.

    Strategies used by bacterial pathogens to cross the blood–brain barrier.
    Le Guennec L, Coureuil M, Nassif X, Bourdoulous S.
    Cell Microbiol. (2020) 22:e13132. Review.

    Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells.
    Ayala-Nunez NV, Follain G, Delalande F, Hirschler A, Partiot E, Hale GL, Bollweg BC, Roels J, Chazal M, Bakoa F, Carocci M, Bourdoulous S, Faklaris O, Zaki SR, Eckly A, Uring-Lambert B, Doussau F, Cianferani S, Carapito C, Jacobs FMJ, Jouvenet N, Goetz JG, Gaudin R.
    Nat Commun. (2019) 27;10(1):4430. 

    Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease.
    Denis K, Le Bris M, Le Guennec L, Barnier JP, Faure C, Gouge A, Bouzinba-Ségard H, Jamet A, Euphrasie D, Durel B, Barois N, Pelissier P, Morand PC, Coureuil Lafont F, M, Join-Lambert O, Nassif Xand Bourdoulous S. 
    Nature Microbiol, (2019) doi: 10.1038/s41564-019-0395-8.

    Molecular interactions between Neisseria meningitidis and its human host.
    Coureuil M, Jamet A, Bille E, Lécuyer H, BOURDOULOUS S, Nassif X.
    Cell Microbiol. (2019) 5:e13063. Review.

    The synergistic effect of chlorotoxin-mApoE in boosting drug-loaded liposomes across the BBB.
    Formicola B, Dal Magro R, Montefusco-Pereira Cv, Lehr Cm, Koch M, Russo L, Grasso G, Deriu Ma, Danani A, Bourdoulous S, Re F.
    J Nanobiotechnology (2019) 11;17(1):115.

    Cannabidiol Increases Proliferation, Migration, Tubulogenesis, and Integrity of Human Brain Endothelial Cells through TRPV2 Activation.
    Luo H, Rossi E, Saubamea B, Chasseigneaux S, Cochois V, Choublier N, Smirnova M, Glacial F, Perrière N, Bourdoulous S, Smadja DM, Menet MC, Couraud PO, Cisternino S, Declèves X.
    Mol Pharm. 2019 Mar 4;16(3):1312-1326.

    An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminans.
    Lécuyer H, Virion Z, Barnier JP, Matczak S, Bourdoulous S, Bianchini E, Saller F, Borgel D, Nassif X, Coureuil M.
    PLoS Pathog. 2018 Apr 9;14(4):e1006981.

    Decoding glycan recognition by bacterial toxins
    Bourdoulous S, Lemichez E.
    Nature Microbiol (2018) 3,124–126.

    Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-Actinin-4.  
    Maïssa N, Covarelli V, Janel S, Simpson N, Bernard SC, Pardo-Lopez L, Durel B, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S,  Bourdoulous S.
    Nature Commun (2017) 1;8:15764.

    A journey into the brain: insight into how bacterial pathogens cross blood-brain barriers.
    Coureuil M., Lecuyer H., Bourdoulous S., X. Nassif. 
    Nature Rev Microbiol (2017) 15(3):149-159.

    Peripheral blood vessels are a niche for blood-borne meningococci.
    Capel E, Barnier JP, Zomer AL, Bole-Feysot C, Nussbaumer T, Jamet A, Lécuyer H, Euphrasie D, Virion Z, Frapy E, Pélissier P, Join-Lambert O, Rattei T, Bourdoulous S, Nassif X, Coureuil M.
    Virulence (2017) 17;8(8):1808-1819.

    Comprehensive Identification of Meningococcal Genes and Small Noncoding RNAs Required for Host Cell Colonization.
    Capel E, Zomer AL, Nussbaumer T, Bole C, Izac B, Frapy E, Meyer J, Bouzinba-Ségard H, Bille E, Jamet A, Cavau A, Letourneur F, Bourdoulous S, Rattei T, Nassif X, Coureuil M.
    MBio. (2016) Aug 2;7(4).

    CD147 is an essential receptor for vascular colonization by Neisseria meningitidis.
    Morand PC, Maïssa N, Bernard SC, Bourdoulous S.
    Med Sci (Paris). (2014) 30:825-7.

    Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonisation.
    Bernard S, Simpson N, Join-LambertO, Federici C, Laran-ChichMP, Maïssa N, Ségard H, Morand P, ChretienF, Taouji S, Chevet E, Janel S, Lafont F, CoureuilM, NiedergangF, MarulloS, CouraudPO, NassifX, Bourdoulous  S.
    Nature Med (2014) 20: 725-731.

    Invasive meningococcal disease: a disease of the endothelial cells.
    Coureuil M, Bourdoulous S, Marullo S, Nassif X.
    Trends Mol Med. (2014) 20:571-8.

    Pathogenesis of meningococcemia.
    Coureuil M, Join-Lambert O, Lécuyer H, Bourdoulous S, Marullo S, Nassif X.
    Cold Spring Harb Perspect Med. (2013) 3: a012393.

    Neisseria meningitidis colonization of the brain endothelium and cerebrospinal fluid invasion.
    Miller F, Lécuyer H, Join-Lambert O, Bourdoulous S, Marullo S, Nassif X, Coureuil M.
    Cell Microbiol. (2013) 15:512-9.

    Mechanism of meningeal invasion by Neisseria meningitidis.
    Coureuil M, Join-Lambert O, Lécuyer H, Bourdoulous S, Marullo S, Nassif X.
    Virulence. (2012); 3:164-72.
    Breaking the wall: targeting of the endothelium by pathogenic bacteria.
    Lemichez E, Lecuit M, Nassif X, Bourdoulous S.
    Nat Rev Microbiol. (2010); 8: 93-104.

    Mechanisms of meningeal invasion by a bacterial extracellular pathogen, the example of Neisseria meningitidis.
    Join-Lambert O, Morand PC, Carbonnelle E, Coureuil M, Bille E, Bourdoulous S, Nassif X.
    Prog Neurobiol. (2010); 2:130-9.

    Meningococcus Hijacks a β2-Adrenoceptor/β-Arrestin Pathway to Cross Brain Microvasculature Endothelium.
    Coureuil M, Lecuyer H, Scott MGH, Boularan C, Enslen H, Soyer M, Mikaty G, Bourdoulous S, Nassif X, Marullo.
    Cell  (2010); 143: 1149-60.

    Meningococcal interactions with the host.
    Carbonnelle E, Hill DJ, Morand P, Griffiths NJ, Bourdoulous S, Marullo S, Nassif X, Virji M.
    Vaccine (2009); 27 S2: B78-89.

     Meningococcal type IV pili recruit the polarity complex to cross the brain endothelium.
    Coureuil M, Mikaty G, Miller F, Lécuyer H, Bernard C, Bourdoulous S, Duménil G, Mège RM, Weksler BB, Romero IA, Couraud PO, Nassif X.
    Science (2009); 3: 83-7.
    JAM-L mediated leukocyte adhesion to endothelial cells is regulated in cis by α4β1 integrin activation.
    Luissint A.C., LutzP,  Calderwood D., Couraud PO and Bourdoulous S.
     J Cell Biol (2008) 183: 1159- 1173.
    PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
    Couty JP, Rampon C, Leveque M, Laran-Chich MP, Bourdoulous S, Greenwood J, Couraud PO.
    J Neurochem. (2007) 103:793-801.
    Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures.
    Doulet N, Donnadieu E, Laran-Chich MP, Niedergang F, Nassif X, Couraud PO and Bourdoulous S.   
    J. Cell Biol (2006) 173:627-637.
    Blood-brain barrier-specific properties of a human adult brain endothelial cell line.
    B. B. Weksler, E. A. Subileau, N. Perrière, P. Charneau, K. Holloway, M. Leveque, H. Tricoire-Leignel, A. Nicotra, S. Bourdoulous, P. Turowski, D. K. Male, F. Roux, J. Greenwood, I. A. Romero and P. O. Couraud. 
    FASEB J. (2005) 19:1872-4.
    Invasion of endothelial cells by Neisseria meningitidis requires cortactin recruitment by a PI3-Kinase/Rac1 signalling pathway triggered by the lipo-oligosaccharide.
    Lambotin M, Hoffmann I, Laran-Chich MP, Nassif X, Couraud PO and Bourdoulous S.
    J. Cell Science (2005) 118: 3805-3816.

    Interactions between Neisseria meningitidis and human cells that promote colonisation and disease.Corbett A, Exley R, Bourdoulous S, Tang CM.
    Expert Rev Mol Med. 2004 Jul 9;6(14):1-14. Review.
    Hydroxyurea downregulates endothelin-1 gene expression and upregulates ICAM-1 gene expression in cultured human endothelial cells.
    Brun E, Bourdoulous S, Couraud PO, Elion J, Krishnamoorthy R and Lapoumeroulie C.
    Pharmacogenomics J. (2003);3(4):215-26.
    JAML, a novel protein with characteristics of a Junctional Adhesion Molecule is induced during differentiation of myeloid leukemia cells.
    Moog-Lutz C., Cavé-Riant F., Guibal F., Breau M.,Di Gioia Y., Couraud PO, Cayre E, Bourdoulous S and LutzP.
    Blood (2003) 102: 3371-3378.
    Microvilli-like structures are associated with the internalization of virulent capsulated Neisseria meningitidis into vascular endothelial cells.
     Eugene E, Hoffmann I, Pujol C, Couraud PO, Bourdoulous S and Nassif X.
    J. Cell Science (2002) 115 : 1231-1241.

    How do extracellular pathogens cross the blood-brain barrier?Nassif X, Bourdoulous S, Eugène E, Couraud PO.
    Trends Microbiol. 2002 May;10(5):227-32. Review.

    Activation of ErbB2 tyrosine kinase receptor supports invasion of endothelial cells by Neisseria meningitidis.
    Hoffmann I, Eugene E, Nassif X, Couraud PO and Bourdoulous S.
    J. Cell Biol (2001) 155:133-143.
    MHC class II engagement in brain endothelial cells induces PKA-dependent interleukin-6 secretion and phosphorylation of cAMP-response element binding protein.
    Etienne S, Bourdoulous S, Strosberg A D and Couraud PO.
    J Immunol (1999) 163 : 3636-41.
    Fibronectin matrix regulates activation of RHO and CDC42 GTPases and cell cycle progression.
    Bourdoulous S, Orend G, MacKenna DA, Pasqualini R and Ruoslahti E.
    J. Cell Biol. (1998) 143: 267-76.
    The C-type lectin domains of lecticans, a family of aggregating chondroitin sulfate proteoglycans, bind tenascin-R by protein-protein interactions independent of carbohydrate moiety.
    Aspberg A, Miura R, Bourdoulous S, Shimonaka M, Heinegard D, Schachner M, Ruoslahti E and Yamaguchi Y.
    Proc Natl Acad Sci U S A. (1997) 94: 10116-21.
    A polymeric form of fibronectin has antimetastatic effects against multiple tumor types.
    Pasqualini R, Bourdoulous S, Koivunen E, Woods VL Jr and Ruoslahti E.
    Nature  Med. (1996) 2: 1197-203.
    Effect of muscular exercise on adoptive experimental autoimmune encephalomyelitis.
    Le Page C, Bourdoulous S, Béraud E, Couraud PO, Rieu M and Ferry A.
    Eur. J. Appl. Physiol.  (1996) 73: 130-5.

    Anergy induction in encephalitogenic T cells by brain microvessel endothelial cells is inhibited by interleukin-1.
    Bourdoulous S, Béraud E, Le Page C, Ferry A, Bernard D, Strosberg AD and Couraud PO.
    Eur. J. Immunol.  (1995) 25: 1176-1183.

    Infection of bovine brain microvessel endothelial cells with Cowdria Ruminantium elicits interleukin-1, -6, and -8 mRNA production and expression of an unusual MHC class II DQ transcript.
    Bourdoulous S, Bensaid A, Martinez D, Sheikboudou C, Trap I, Strosberg AD and Couraud PO.
    J. Immunol, (1995) 154: 4032-4038.
    Catecholamines stimulate MHC class I, class II and invariant chain gene expression in brain endothelium through different mechanisms.
    Bourdoulous S, Durieu-Trautmann O, Strosberg AD and Couraud PO.
    J. Immunol. (1993) 150: 1486-10495
    Immortalized rat brain microvessel endothelial cells: II-Pharmacological characterization.
    Durieu-Trautmann O, Bourdoulous S, Roux F, Bourre J M, Strosberg AD and Couraud PO.
    Adv. Exp. Med. Biol. (1992).331: 205-210.